Introduction the biodegradation of petroleum and other hydrocarbons in the environment is a complex process, whos quantitative and qualitative aspects depend on the nature and amount of the oil or hydrocarbons present, the ambient and seasonal environmental. Isolation, identification and characterization of oil degrading bacteria isolated 435 4. A survey of petroleum degrading bacteria was carried out in the indian part of deltaic sunderbans to evaluate the distribution of the naturally occurring petroleum degrading aerobic bacteria. About 50ml medium was dispensed in 250ml conical flasks. Hydrocarbondegrading bacteria and the bacterial community.
These bacteria were screened with two important factors such as growth rate on crude oil and hydrocarbon biodegradation, and then three strains were selected from 15 isolated strains for further study. The number of oil degrading bacteria was relatively similar in the two environments, except that in the sediments of restored wetland, which exceeded up to several times the number of oil. The oil degrading bacteria and other microorganisms degraded the chemical substance this process is termed as bioremediation. Several bacteria are even known to feed exclusively on hydrocarbons 39. Phylogenetic analysis of oildegrading bacteria associated with polluted sites in river state, nigeria. This strain is capable of efficient degradation of components of mineral oil alkanes, isoalkanes, and alkyl residues of the naphthene and arene fraction. Isolation, screening and activity of hydrocarbondegrading bacteria. Many indigenous microorganisms in water and soil are capable of degrading.
Biodegradation by indigenous bacteria represents one of the primary mechanisms by which oil pollutants can be removed from the environment. Isolation and characterisation of crude oil sludge degrading. Dispersant and dispersant oil treated samples increased the abundance of colwellia from 1% to 2643. Hydrocarbondegrading microorganisms are ubiquitous in the marine environment 27, 76. The timing of hydrocarbon charges and the postcharge temperature history of the reservoir can have major effects on oil quality. The strain bears stably inherited plasmids of sizes 120, 9, and 8 kb, which can. Isolation and identification of oil degrading bacteria from oil sludge. Elghazawani2 and ahmed issa3 1microbiology laboratory, botany department, omar almukhtar university, elbeida libya 2inspection and corrosion section, sarir oil field, arabian gulf oil company, benghazi, libya. Pdf in developing nations like india pesticides play an important role in agricultural. Pdf isolation of pesticide degrading microorganisms from soil. Isolation and characterization of oil degrading bacteria from. Oil fields occur typically at several thousand metres below ground and, at temperatures of 6090c, are considered sterile. Microbial degradation of petroleum hydrocarbon contaminants.
Treatment of hydrocarbonrich wastewater using oil degrading. Isolation, identification and degradation characteristics. We cultivated subcultures of four strains of hydrocarbondegrading strains isolated from oilpolluted wastewater of american petroleum. Isolation, identification and characterization of oil. A total of six pure cultures, which could utilize crude oil as the sole carbon and energy source, were isolated on the lb agar slants under similar conditions. Oil dispersants hinder oildegrading bacteria microbiology.
N, isolation and identification of petroleum hydrocarbon degrading bacteria from oil contaminated soil samples the main factors that affect the effect of flasks and add one gram of hydrocarbon such as remediation include the ph value, soil moisture, benzene, toluene and xylene. Aug 15, 2011 the natural background rates of oil biodegradation initially were estimated at 1. This research was study on degradation of plastic films in solid and liquid media, concentration of optical density od analysis, and concentration of cell dry g analysis. Dec 29, 2018 the growth and isolation of oil degrading bacteria with morphological differences were performed on msm containing crude oil as the only energy and carbon source. Article history received may 2015 accepted 20 august 2015 abstract twentythree crude oil degrading bacteria were isolated from oil contaminated sites near the red sea. A new marine oil degrading consortium ctod was isolated from constanta seawater using 5% vv fuel oil, as the sole carbon source. Isolation and characterisation of crude oil sludge degrading bacteria linda u. Bacteria are the most active agents in petroleum degradation, and they work as primary degraders of spilled oil in environment 37, 38. Isolation of bacteria five gramoil contaminated soil sample was inoculated in the 250 ml erlenmeyer flask of 100 l oil medium on a horizontal shaker 150 rm pm at 35. Jun 26, 2017 the deepwater horizon oil spill in the gulf of mexico in 2010 is one of the most studied spills in history, yet scientists havent agreed on the role of microbes in eating up the oil.
Oil biodegradation and oildegrading microbial populations in. The origin of oil and gas industry in india can be traced back to the year 1867 when. Chemical dispersants enhance the activity of oil and gas. Isolation and characterization of hydrocarbondegrading. Isolation and characterization of bacteria degrading n. Bacteria enriched from surface and plume waters of the 2010 deepwater horizon oil spill show that the combined capabilities of communitywide hydrocarbon degradation is greater than its individual.
In the present study, altogether 32 morphologically distinct bacteria were isolated from crude oil contaminated soil and 18 different hydrocarbon degrading bacteria were number hydrocarbon degrading bacterial strain from and. Pdf indigenous pahdegrading bacteria from oilpolluted. Beef peptone liquid medium, beef peptone solid medium, according to reference. Screening of hydrocarbon degrading bacteria isolated from. Isolation, screening and activity of hydrocarbondegrading. During biodegradation, microbial organisms metabolize petroleum, degrading the hydrocarbon content. Crude oil and petroleum is a complex mixture of hydrocarbons, which cause widespread environmental contaminants following a spill. In this study, a consortium of phototrophic microorganisms and a bacterium is developed on the discs of a rotating biological contactor rbc for treatment of wastewater containing diesel oil. Concentrations of naturally occurring oildegrading bacteria during this period were 15. Thus, in order to get insight of the bioremediation process of hydrocarbons in oil contaminated environments of ouagadougou city, the present study focused on the isolation, characterization and identification of indigenous hydrocarbon degrading bacterial strains with regards to the conditions for optimizing their activities and. Altogen labs has developed active bioremediation technology based on natural oildegrading bacteria for largescale remediation of hydrocarboncontaminated water and soil. The natural background rates of oil biodegradation initially were estimated at 1. Similarly, in preliminary study 57 oil degrading bacteria were isolated from oil impregnated soil in search of potent hydrocarbon degrader 14.
The acinetobacter calcoaceticusstrain tm31 has been isolated from a microbial assemblage of a pilot plant that purifies waste water polluted with mineral oil. Isolation of pesticide degrading microorganisms from soil. Alsaleh department of biological science, kuwait university, kuwait abstract the planning of cities must insure a balance between human consumption and. Isolation and molecular identification of hydrocarbon degrading bacteria from oil contaminated soil fatiha dilmi 1, 2, abdelwaheb chibani 1, khadidja senouci rezkallah 2 1laboratory of microbiology and plant science, department of biology, faculty of natural and life sciences, university of abdelhamidibn badis, mostaganem, algeria. Deepsea oil plume enriches indigenous oildegrading bacteria. Abstract the role of plasmidborne genes in the biodegradation of chevron escravos crude oil by bacteria was determined. N, isolation and identification of petroleum hydrocarbon degrading bacteria from oil contaminated soil samples the main factors that affect the effect of flasks and add one gram.
In fact, this represents the main origin of failure of most of the bioremediation applications for cleaning polluted areas with hydrocarbons. Isolation and identification of hydrocarbon degrading. May 15, 2014 fifteen crude oil degrading bacteria were isolated from oil contaminated sites in the persian gulf at khorramshahr provenance. Atlas, department of biology, university of louisville, louisville kentucky 40292, united states. Characterization of crude oildegrading bacteria in a crude. Phylogenetic analysis of oildegrading bacteria associated. Studies on crude oil degrading bacteria isolated from libyan desert farag m. Study of plastic degrading bacteria by aneurinibacillus. Oct 14, 2016 the objectives were study of bacteria aneurinibacillus migulanus that can degrading plastic packaging and efficacy of degradation. Conclusion environment pollution caused by released of a wide range of compound as a consequence of industrial progress has assumed serious proportions.
Characterization of crude oil degrading bacteria in a crude oil contaminated and uncontaminated site in kuwait a. Lipids and fatty acids from plants, animals and microbes and the products of their conversion in anoxic. Reconstructing metabolic pathways of hydrocarbondegrading. The presence of oildegrading bacteria is a prerequisite for oil degradation to occur. Floodgate 36 listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi which were isolated.
Feb 06, 20 sediments samples were collected once in a month for a period of twelve months from area surrounding ennore creek and screened for hydrocarbon degrading bacteria. Pdf extraction and characterization of oil degrading bacteria. The aim of our study is the isolation and identification of petroleum hydrocarbon degrading bacteria from oil contaminated soil samples. For the treatment of waste and waste water the use of the oil degrading bacteria has been strongly applied in controlled systems hazen, et, al, 2010. Recent studies have identified bacteria from more than 79 genera that are capable of degrading petroleum hydrocarbons tremblay et al. Two bacteria, strains ibbct6 and ibbct7 were isolated from the ctod consortium. Pdf biodegradation of crude oil using local bacterial isolates. Pdf hydrocarbon degrading microorganisms were isolated from ten oil. Degradation of mineral oil with a strain of acinetobacter. Pdf the present study was conducted to determine the isolation, identification and characterization of efficient oil degrading bacterial strains. Petroleum hydrocarbondegrading bacteria for the remediation of. Parametric optimization of media for the crude oil degrading. Studies on crude oil degrading bacteria isolated from libyan.
Isolation and molecular identification of hydrocarbon. Isolation and identification of oil degrading bacteria from. Alcanivorax borkumensis was the first hcb to have its genome sequenced. Isolation and characterization of crude oil degrading. Of the 1 crude oil degrading isolates 15 isolates were selected and cultivated in bh media with 1% crude oil as a sole carbon and energy source.
Isolation of hydrocarbon degrading bacteria from soils. Highly saline formation waters may inhibit bacterial degradation and effectively shield oils from oilquality deterioration. To prevent development of hazardous waste the process of bioremediation has been followed. Oil degradation in soil 523 in a more recent publication, francke and. Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbon degrading activities of microbial communities, in particular by a recently discovered group of specialists, the hydrocarbonoclastic bacteria hcb. The report is available in electronic format pdf at. The aim of this study was isolation and characterization of new marine oil degrading bacteria from a seawater sample. Microbial degradation of petroleum hydrocarbons microbiology. Enrichment cultures and isolation procedure alpha subunit of the dioxygenase from pahbiodegradation pathway were characterized in intertidal sediments of patagonia in order to enrich selectively for pahdegrading bacteria. Species of colwellia are capable of degrading both hydrocarbons oil and dispersants, while marinobacter mainly degrade hydrocarbons.
The process can take more or less time depending on the amount of type and amount of bacteria, the reservoir or ecosystem in which the bacteria are found, and the amount of oxygen present. Adeleke1,3,4 background high demand for petroleum products all over the world has led to an increase in crude. Aug 11, 2017 application of chemical dispersants to oil spills in the marine environment is a common practice to disperse oil into the water column and stimulate oil biodegradation by increasing its. For examining the degradation of oil, bushnell haas medium bhm supplemented with 5g50ml of petrol, diesel and used engine oil to be used 11.
Arctic marine potential of microbial oil degradation aarhus universitet. Assay of crude oil degradation in liquid medium the crude oil degradation capability of both bacterial strains was analyzed by liquid medium. Twentyfive crude oil degrading bacteria were isolated from oil contaminated sites in the persian gulf and the caspian sea. Dispersants set the stage for oil degrading bacteria. Isolation and characterization of crudeoildegrading.
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